Shelf Stocking Protocol

General

• Make sure items labeled with older dates are in the front so they can be used first.
• Plates without antibiotics in the cold room can last for about a year; plates with antibiotics can last about 3 months.
• If liquid media is cloudy, it is contaminated and needs to be disposed.
• Regularly check media and throw out anything that looks contaminated or is too old

Quantities to keep stocked

Sterile Media, Reagents, & Buffers (Room Temp)

| Item | Quantity | |:—————————-|:—————-| |CPG broth, 100 ml |5-10 bottles | |CPG broth, 500 ml |4-6 bottles | |CPG agar, 500 ml |4-6 bottles |

Item	Quantity
LB broth, 100 ml	4-8 bottles
LB agar, 100 ml	1-4 bottles
LB agar, 250 ml	5-10 bottles

Item	Quantity
Glycerol 50%, 100 ml	3-10 bottles
TZC 1%, 50 ml	1-4 vials

Sterile solids

| Item | Quantity | |:—————————-|:—————-| |tips 1-20 ul |6-10 boxes | |tips 2-200 ul |15-40 boxes | |tips 2-1000 ul |10-20 boxes | |tips LTS style 1250 ul |4-10 boxes |

Item	Quantity
microfuge tubes, 0.5 ml	2-4 pint jars
microfuge tubes, 1.7 ml	4-8 quart jars 2-6 pint jars
microfuge tubes, 2.0 ml	2-4 quart jars 1-3 pint jars

Item	Quantity
test tubes	2-4 racks
flasks, 125 ml	10 minimum. When there is time, sterilize them all
flasks, 250 ml	4-8
flasks, 500 ml	5

Item	Quantity
glass petri dishes	as many as washed
glass beads 4.5 mm	5-10 bottles
toothpicks	2-4 containers
wooden sticks	10-30 tubes
sterile swabs	5-10 tubes

Nonsterile Reagents & Buffers (Room Temp)

| Item | Quantity | |:—————————-|:—————-| |Tris 50 mM pH 8.0, 50 ml |2-5 | |EDTA 0.5 M, pH 8.0, 100 ml |1 bottle | |50x TAE Buffer, 1 L |remake when < 100 ml remains|

Sterile Reagents & Buffers (-20 C)

Item	Quantity
IPTG, 0.5 ml	4-20 1.5 ml tubes
XGal, 10 ml	<500 ul remaining Note below
Antibiotic stocks	4-20 1.5 ml tubes

Note: XGal and reagents with hazardous solvents need to be prepared by experienced lab members (i.e. most likely not Hourly workers)