protocols

Lab protocols for the Lowe-Power lab (scroll down for table of contents Readme)

View the Project on GitHub lowepowerlab/protocols

Genome sets for comparative genomics

Narrative: RSSC Phylogenomics

Tiffany maintains this narrative with all of the publicly available RSSC genomes.

https://narrative.kbase.us/narrative/123807

Useful genome sets:

Narrative: Burkholderiales Species Representatives

Tiffany used GTDB to identify genome representatives of approx 300 species in the Burkholderiales order. All genomes were described as complete on GTDB, but it is possible that there are mistakes. The genomes are named according to GTDB taxonomy when the narrative was created in Summer 2023.

https://narrative.kbase.us/narrative/142785

Useful genome set:

Narrative: Other Ralstonia phylogenomics

Tiffany maintains this narrative with all of the publicly available Ralstonia genomes that are not RSSC.

https://narrative.kbase.us/narrative/127696

Narrative: Cupriavidus phylogenomics

Tiffany maintains this narrative with all of the publicly available Cupriavidus genus genomes.

https://narrative.kbase.us/narrative/142720

Narrative: Pandoraea phylogenomics

Tiffany maintains this narrative with all of the publicly available Pandoraea genus genomes.

https://narrative.kbase.us/narrative/142975

Transcriptomics

Quorum sensing transcriptome datasets from multiple sources:

efpR transcriptome from Perrier et al.

Phyl. IIB-1 strain UY031 in potato stems and rich media from de Pedro Jove 2020

Phyl. I strain RS-SY1 in pepper stems from Du et al 2022.

Phyl. IIB-4 strains IBSBF1503 and UW163 in tomato, banana, and melon stems from Ailloud et al. 2016

Phyl. IV-BDB strain UGMSS-Db01 in culture. (The in planta transcriptome had almost no bacterial reads)